Invalidity of the acetylene reduction assay in alkane-utilizing, nitrogen-fixing bacteria
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منابع مشابه
Invalidity of the acetylene reduction assay in alkane-utilizing, nitrogen-fixing bacteria.
The cause of the failure of the C2H2-C2H4 assay for nitrogen-fixing bacteria growing on lower alkanes was studied. Acetylene was a strong competitive inhibitor of methane oxidation for methane-utilizing bacteria, as well as for the oxidation of lower alkanes by other bacteria, so that energy and reducing power were no longer available for the reduction of acetylene by nitrogenase. Nitrogen-fixi...
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Nitrogen-fixing activity associated with different wetland rice varieties was measured at various growth stages by an in situ acetylene reduction method after the activities of blue-green algae (cyanobacteria) in the flood water and on the lower portion of the rice stem were eliminated. Nitrogen-fixing activities associated with rice varieties differed with plant growth stages. The activities i...
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Nitrogen fixation in waterlogged, soil-straw, and sandday-straw mixtures was measured with the C2H2reduction assay, the I5N-tracer technique, and the Kjeldahl method. The reduction of 6 to 15 moles C2H2 corresponded to the fixation of 1 mole N2. The theoretical ratio is 3 moles C2H2 to 1 mole N2. A ratio of 3 moles C2H2 reduced for each mole of N2 fixed was obtained when samples of sandxlay-str...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1976
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.31.5.640-647.1976